Day 2 :
Keynote Forum
Sibel Silici
Erciyes University, Turkey
Keynote: Role of propolis ın histological changes of LPS induced renal damage
Time : 11:00-11:40
Biography:
Abstract:
In recent years, propolis has been the object of extensive research for its antibacterial, anti-oxidant, anti-infl ammatory,\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nand anti-tumoral activities. Th is study aimed to determine the nephroprotective effi ciency of propolis on experimental\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nendotoxemic renal damage in rats. In the current study, fi ft y adult Sprague Dawley rats (weighing 200-300 g) were randomly\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\ndivided into fi ve groups of ten rats each. Normal saline solution was administered to the rats in the control group, while in the\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nsecond group LPS (30 mg/kg), in the third group propolis (250 mg/kg), in the fourth group fi rst propolis and then LPS (30 mg/\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nkg), and in the fi ft h group, fi rst LPS (30 mg/kg) and then propolis were given. Six hours aft er the application, histopathological\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nchanges in the kidney tissue samples were determined. Besides, MDA was analyzed as an oxidative stress marker. Th e results demonstrated that the application of propolis prior to LPS administration did not have a signifi cant protective effect against LPS-induced renal damage. In comparison with the kidney sections of rats treated with only LPS, administration of propolis showed a mild but not a signifi cant eff ect against LPS-induced renal damage.
- Urologic Oncology
- Genitourinary Medicine
Session Introduction
Amal Fawzy Said
Cairo University, Egypt
Title: Increased Cell-Free Circulating Plasma DNA in cancer Prostate
Biography:
Amal Fawzy Said is an assistant professor from Cairo University, Egypt
Abstract:
BACKGROUND: Prostate Cancer is the most common cancer overall after breast cancer, and is the most common cancer affecting men, who have a lifetime risk 10% of developing the disease and 3% chance of dying. DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell- free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe..
Aim of work: to determine the role of total plasma cell-free DNA levels in newly diagnosed cancer prostate.
Methodology: The amount of DNA was determined by a quantitative real-time PCR technique, using two sets of primers to amplify the consensus ALU sequence. ALU 115-bp amplicons were representing the total amount of free cell-free circulating DNA. While ALU 247-bp amplicons representing the DNA released from non-apoptotic cells. DNA integrity was calculated as the ratio of concentrations in each assay.
Results: The levels of plasma cell-free DNA in the cancer group were significantly higher in comparison with the benign tumor group (P < 0.001) and the healthy control group (P < 0.001). There was statistically significant association with some prognostic parameters
Xiaotian Yuan
Karolinska University Hospital, Sweden
Title: Differential frequencies in TERT promoter and FGFR3 mutations between Chinese and western patients with urothelial bladder cancer
Biography:
Xiaotian Yuan is an PhD student from Karolinska University Hospital, Sweden
Abstract:
The TERT promoter and FGFR3 gene mutations are two most common genetic events in urothelial bladder cancer (UBC) and these mutation assays in patient urine have been shown promising biomarkers for UBC diagnosis and surveillance based on analyses of UBC patients from western countries. However, we observed TERT promoter and FGFR3 mutations in 87 of 182 (47.8%) and 7 of 102 (6.7%) Han Chinese patients with UBC, respectively, which were lower than those in UBC patients from western countries. In 46 urine samples from patients with TERT-promoter mutation-carrying tumors, the mutant promoter was detected in 24 (52%) prior to operation and disappeared in 80% urine samples one week post-operation. TERT mRNA was detected in urine derived from 46 of 49 (94%) of patients determined before operation independently of TERT promoter mutations. Collectively, FGFR3 mutations occur at a very low rate in Han Chinese UBC patients compared with western patients, indicating a different aetiology between Chinese and western UBCs. Han Chinese UBC patients have a relatively lower TERT promoter mutation frequency, and simultaneous detection of both mutant TERT promoter and TERT mRNA improves sensitivity and specificity of urine-based diagnosis.
Emanuele Corongiu
University of Rome, Italy
Title: Role of percutaneous tibial nerve stimulation in the treatment of neurogenic overactive bladder syndrome.
Time : 12:40-13:10
Biography:
Emauele Corongiu is an professor from University of Rome, Italy
Abstract:
Overactive bladder syndrome (OAB) is characterized by a series of urinary symptoms such as incontinence, urgency, frequency, nocturia and it is nowadays differentiated in OAB, due to an involuntary urodynamic detrusor contraction, responsible of the symtoms and Neurogenic Detrusor overactivity (NDO). Many neurologic pathologies can be responsible of NDO such as Parkinson disease, multiple sclerosis, spinal cord injury, cerebral palsy, brain tumors, cerebrovascular pathologies, and meningomyelocele and the lower urinary tract disfunction depend on the site and the completeness of the associated neurological lesions. Aim of the study is to evaluate preliminary results on symptoms and urodynamic findings of posterior tibial nerve stimulation (PTNS) in patients with NDO.
METHODS:
Twelve patients (pts.), 9 men and 3 females, age ranged from 25 and 76 years with clinical and urodynamic diagnosis of NDO were included in the study. Electrical stimulations were applied unilaterally from the medial malleolus and posterior to the edge of the tibia twice a week for a total of 6 weeks. Urodynamic parameters were evaluated before and after treatment. Overactive bladder questionnaire (AOB-q) and voiding diary were performed in all pts. before treatment after 6 elettrical stimulations and at the end of treatment.
RESULTS:
PTNS was found to be effective on OAB symtoms in all pts., according to the data of the questionnaires and voiding diary. Mean 1st involuntary detrusor contraction (IDC) and mean maximum cistometric capacity(MCC) were significantly improved on standard cystometry. 8 pts. (66,6%) showed a complete disappearance of IDC and the other 4pts.(33,3%) showed an increased filling volume to IDC. No complications have been reported and pts. compliance to treatment was very high.
CONCLUSIONS:
PTNS is an effective and safe option to treat pts. with symptoms from NDO. Further studies are needed to assess the role of this technique, and to establish the length of treatment in order to maintain the results obtained.
Samileh Noorbaksh
Research Center of Pediatric Infectious Diseases, Iran
Title: Dynamic magnetic resonance urography vs DMSA scan, IVP, Ultrasonography in children with urinary tract infection
Time : 14:00-14:30
Biography:
Samileh Noorbaksh is an Professor Research Center of Pediatric Infectious Diseases, Iran
Abstract:
Background: Urinary tract infection 0 in children causes renal scarring and permanent
damage to the organ. In this study, we compared the diagnostic value of magnetic
resonance urogram for urinary tract anomalies with other conventional imaging
methods in children with UTI.
Methods: In this case-control study, 190 children (mean age 3.23±3.59 yrs) with UTI
were recruited from the Pediatric Ward of Rasul-e-Akram Hospital during 2007-2009.
The patients were divided into two groups based on the applied imaging technique:
MRU (cases) and conventional imaging groups (controls).
Results: Abnormal imaging detection rates for Ultrasonography were 32%, X-ray of
kidneys, ureters and bladder (KUB) 9%, Intravenous Pyelogram (IVP) 26%, Voiding
Cystoure therogram (VCUG) 54%, Dimercaptosuccinic Acid scan (DMSA) indicating
non-obstructive (reflux) uropathy in 76% (mean age 3.5 yrs) and MRU 43% (mean age
1.6 yrs), respectively. A meaningful correlation was observed between MRU and DMSA
scan with IVP results (Kappa=0.75). KUB and Ultrasonography had similar results in
cases with abnormal MRU and DMSA scan (P=0.121). MRU had strong agreement with
VCUG and IVP for the detection of obstructive uropathy and scar due to congenital
malformation even during intrauterine life but not with sonography results.
Conclusion: Sonography had poor results for the diagnosis of urinary tract anomalies in
comparison with MRU. Use of dynamic MRU for the diagnosis of congenital anomalies
(hydronephrosis, obstruction, pyelonephritis, renal scar) in children with UTI seems to
be of better help, although higher costs and the need for sedation during MRU still are
its disadvantages.
Chetan Mehndiratta
Ranbaxy Laboratories Ltd., India
Title: Effect of silodosin and dutasteride combination treatment in Indian patients of benign prostate hyperplasia with an enlarged prostate and co-morbid condition
Time : 11:40-12:10
Biography:
Chetan Mehndiratta is an researcher from Ranbaxy Laboratories Ltd., India
Abstract:
Introduction: Benign prostate hyperplasia can occur up to about 50% of men by age 50 with an increase in incidence with age. Th e International Prostate Symptom Score (IPSS) questionnaire can help in the diagnosis and determine patient outcome. Th e addition of dutasteride, a 5-alpha-reductase inhibitor to silodosin can be benefi cial in cases of benign prostate hyperplasia with an enlarged prostate especially in patients with co-morbid conditions like hypertension, obesity and dyslipidemia. Objective: Th is study has evaluated the eff ectiveness and safety of combination treatment of silodosin and dutasteride in Indian patients with mild to severe benign prostatic hyperplasia symptoms. Methods: Th e study was a non-interventional observational study, conducted in 1695 Indian male patients with benign prostatic symptoms between 45-75 years of age. All the patients received a combination of silodosin and dutasteride for benign prostatic hyperplasia with enlarged prostate. Th e study results were the treating physician’s observations based on which descriptive analysis was carried out. Results: Around 71% of the patients in this study were of more than 55 years of age. Th e study observed that at baseline, majority (57%) of patients had moderate prostatic hyperplasia whereas at the end of the study aft er receiving combination of silodosin and dutasteride, 70% of patients had mild prostatic hyperplasia. Th e combination of silodosin and dutasteride brought an overall improvement in symptoms and symptom in 79% patient and prevented aggravation of symptoms in 20% patients. Th e patients with co-morbid conditions in the study were 36% with hypertension, 15% with obesity, and 10% with dyslipidemia. Less than 2% patients reported adverse events with the treatment. Conclusion: Th e combination of silodosin and dutasteride was found to be effi cacious and safe by improving the symptoms in patients of benign prostate hyperplasia with enlarged prostate, especially in comorbid conditions like hypertension, obesity and dyslipidemia.
Jacques Choucair
Saint Joseph University, Lebanon
Title: Ertapenem as treatment for ESBL producing E. coli prostatitis
Time : 12:10-12:40
Biography:
Jacques Choucair is an professor from Saint Joseph University, Lebanon
Abstract:
Introduction: Prostatitis presents one of the most common entities encountered in urologic practice. Treating ESBL producing enterobacteriaecea urinary tract infections is a major problem especially in prostatitis because of the absence of active transporters for antibiotics and a relatively low prostate penetration of these drugs. Th us, therapeutic options are limited in infections with such resistant bacteria and require the use of carbapenems in the majority of the cases. Ertapenem is used in acute bacterial prostatitis yet not been approved by the FDA for this indication. Th e goal of this study is to compare the effi cacy of ertapenemas to other antibiotics in the treatment of prostatitis with ESBL producing E. coli. Methods: It is a comparative retrospective study of the fi les of patients admitted in Hotel Dieu de France between 1st July 2008 and 1st July 2014 treated for acute bacterial prostatitis caused by E. coli producing ESBL with ertapenem and other antibiotics. Results: Th e mean age of the 110 patients of this study was 69.58 years. Ertapenem was administered without previous effi cient antibiotic treatment in 34 cases. 18.18% recurred in a period of three months. 85% of recurrences were caused by ESBL producing E. coli (15.45%). 17% and 19% of patients treated with meropenem and imipenem respectively recurred in a 3 months period. Conclusion: Ertapenem is as eff ective as other carbapenems in treatment of E. coli producing ESBL and is not associated with a higher recurrence rate.
Ahmed Hashim
Ain Shams University School of Medicine, Egypt
Title: The coagulation depth of bipolar plasma vaporization of the prostate vs bipolar resection of the prostate vs transurethral resection of the prostate
Time : 12:40-13:10
Biography:
Ahmed Ezat Hashim has completed his Ph.D at the age of 23 years from Ain-Shams University and M.Sc. in Urology in 2012 from Ain-Shams University. He had been a resident of Urology in K. El-Koba Military hospital for 2 years and then Ahmed Maher Educational Hospital for 2 years. He is a Specialist of Urology in Wady El-Nile General Intelligence Hospital and in Manshyet El-Bakry Governmental Hospital since 2013 in parallel with working as a doctor (Medical Guidance and Insurance) for a Petroleum Company PetroDara since 2010.
Abstract:
In aim of comparing the coagulation depth in tissue specimen following Bipolar plasma vaporization of the prostate (BPVP), Bipolar resection of the prostate and Transurethral resection of the prostate (TURP) procedures. Because of the conventional transurethral resection of the prostate (TURP) is regarded as the gold standard in surgical treatment of benign prostatic hyperplasia (BPH) because of its immediate and enduring effi cacy. However, problems that have not yet been overcome for TURP include absorption of irrigation fl uid and bleeding. For this reason, alternative surgical options, such as bipolar transurethral resection of the prostate (BTURP) and bipolar transurethral vaporization of the prostate (BPVP) have been developed. In our study which done on 10 patient’s prostate, taking a diff erent 3 chips aft er the diff erent 3 techniques; bipolar plasma vaporization of the prostate, bipolar trans urethral resection of the prostate and the classic trans urethral resection of the prostate, were applied for a diff erent 3 areas of the prostate, comparing the coagulation depth of it pathologicaly. In our study the coagulation depth induced by BPVP was greater than that induced by BTURP and TURP, with no signifi cant diff erence between BPVP and BTURP (p > 0.05), while with a signifi cant diff erence between BPVP and TURP (p < 0.05). And the coagulation depth induced by BTURP was greater than that induced by TURP but with no signifi cant diff erence (p > 0.05). What prove that there is slight signifi cant diff erence in the coagulation depth of novo BPVP and the coagulation depth of the classic TURP.