Urology

Objective: To determine the effectiveness of intralesional application of onabotulinum toxin A in patients with Peyronie disease.\r\n\r\nMaterials & Methods: A prospective therapeutic cohort study was undertaken in patients ≥18 year with stable disease were included. Intervention: One-time intralesional application of 100 IU of onabotulinum toxin A. We included 22 patients from Urology consult from october 1st 2011 to june 30th 2012. Primary outcome measure: Grade of curvature. Secondary outcome measures: Thickness of the fibrous plaque, erectile dysfunction improvement and pain. Statistical analysis included the Pearson chi-square test for categorical variables and the student t test for quantitative variables. Any p value <0.05 was considered statistically significant.\r\n\r\nResults: The size of the fibrous plaque was reduced from 0.34±0.20 cm to 0.27±0.13 cm after treatment (p=0.014). The curvature initially averaged 32.95±9.21°, improving to 25±9.38° (p=0.025). According to the Kelami classification, the curvature was <30° in 14 cases (63.6%) and was 30–60° in 8 cases (36.4%). At 16 wk, the curvature was <30° in 19 cases (86.4%) and 30–60° in 3 cases (13.6%). The erectile dysfunction grade was 16.18±4.46 before treatment and 18.22±4.55 after treatment (p=0.002). Pain was reduced from 3.36±3.48 before treatment to 1.14 1.58 after treatment (p=0.001). \r\n\r\nConclusions: The application of onabotulinum toxin A can improve the clinical manifestations of Peyronie disease due to fibrosis, increasing sexual function in affected patients.\r\n\r\n\r\n

Objective: Telomeres are specialized terminal capping structures at the end of eukaryotic chromosomes composed of TTAGGG repeats and a variety of proteins such as shelterin and telomerase. There are some telomere related diseases including male infertility that reveal the importance of repeat size in the disease occurrence.rnrnAim: In this research study, we intend to explore possible relationship between leukocyte telomere lengths with azoospermia.rnrnMethods: In this case control study, peripheral blood was taken from 30 idiopathic infertile and 30 healthy and fertile men referred to several infertility clinics in Tehran. Following to DNA extraction, telomere length was determined by a quantitative real-time PCR-based method using specific primers to the telomere region and a single copy housekeeping gene. The relative telomere length was measured according to a comparison between T/S ratios in patients and controls. The results were analyzed by SPSS and REST software.rnrnResults: Interestingly, the relative leukocyte telomere length (T/S) of Azoospermic men was found to be significantly lower (95% CI, p=0.000) when compared to controls (0. 54 vs. 0.84) meaning telomere length in the case group was significantly lower than those of the control group (95% CI, p=0.000).rnrnConclusion: Blood could be an informative and easy access tissue to study telomere size as a new genetic marker for determining a group of men with infertility problem due to the determinant. The same investigation using a bigger population of patient- control will evaluate the present findings.rn