Day 2 :
Erciyes University, Turkey
Time : 11:00-11:40
In recent years, propolis has been the object of extensive research for its antibacterial, anti-oxidant, anti-infl ammatory,\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nand anti-tumoral activities. Th is study aimed to determine the nephroprotective effi ciency of propolis on experimental\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nendotoxemic renal damage in rats. In the current study, fi ft y adult Sprague Dawley rats (weighing 200-300 g) were randomly\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\ndivided into fi ve groups of ten rats each. Normal saline solution was administered to the rats in the control group, while in the\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nsecond group LPS (30 mg/kg), in the third group propolis (250 mg/kg), in the fourth group fi rst propolis and then LPS (30 mg/\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nkg), and in the fi ft h group, fi rst LPS (30 mg/kg) and then propolis were given. Six hours aft er the application, histopathological\\\\\\\\\\\\\\\\r\\\\\\\\\\\\\\\\nchanges in the kidney tissue samples were determined. Besides, MDA was analyzed as an oxidative stress marker. Th e results demonstrated that the application of propolis prior to LPS administration did not have a signifi cant protective effect against LPS-induced renal damage. In comparison with the kidney sections of rats treated with only LPS, administration of propolis showed a mild but not a signifi cant eff ect against LPS-induced renal damage.
- Urologic Oncology
- Genitourinary Medicine
Cairo University, Egypt
Amal Fawzy Said is an assistant professor from Cairo University, Egypt
BACKGROUND: Prostate Cancer is the most common cancer overall after breast cancer, and is the most common cancer affecting men, who have a lifetime risk 10% of developing the disease and 3% chance of dying. DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell- free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe..
Aim of work: to determine the role of total plasma cell-free DNA levels in newly diagnosed cancer prostate.
Methodology: The amount of DNA was determined by a quantitative real-time PCR technique, using two sets of primers to amplify the consensus ALU sequence. ALU 115-bp amplicons were representing the total amount of free cell-free circulating DNA. While ALU 247-bp amplicons representing the DNA released from non-apoptotic cells. DNA integrity was calculated as the ratio of concentrations in each assay.
Results: The levels of plasma cell-free DNA in the cancer group were significantly higher in comparison with the benign tumor group (P < 0.001) and the healthy control group (P < 0.001). There was statistically significant association with some prognostic parameters
Karolinska University Hospital, Sweden
Xiaotian Yuan is an PhD student from Karolinska University Hospital, Sweden
The TERT promoter and FGFR3 gene mutations are two most common genetic events in urothelial bladder cancer (UBC) and these mutation assays in patient urine have been shown promising biomarkers for UBC diagnosis and surveillance based on analyses of UBC patients from western countries. However, we observed TERT promoter and FGFR3 mutations in 87 of 182 (47.8%) and 7 of 102 (6.7%) Han Chinese patients with UBC, respectively, which were lower than those in UBC patients from western countries. In 46 urine samples from patients with TERT-promoter mutation-carrying tumors, the mutant promoter was detected in 24 (52%) prior to operation and disappeared in 80% urine samples one week post-operation. TERT mRNA was detected in urine derived from 46 of 49 (94%) of patients determined before operation independently of TERT promoter mutations. Collectively, FGFR3 mutations occur at a very low rate in Han Chinese UBC patients compared with western patients, indicating a different aetiology between Chinese and western UBCs. Han Chinese UBC patients have a relatively lower TERT promoter mutation frequency, and simultaneous detection of both mutant TERT promoter and TERT mRNA improves sensitivity and specificity of urine-based diagnosis.
University of Rome, Italy
Title: Role of percutaneous tibial nerve stimulation in the treatment of neurogenic overactive bladder syndrome.
Time : 12:40-13:10
Emauele Corongiu is an professor from University of Rome, Italy
Overactive bladder syndrome (OAB) is characterized by a series of urinary symptoms such as incontinence, urgency, frequency, nocturia and it is nowadays differentiated in OAB, due to an involuntary urodynamic detrusor contraction, responsible of the symtoms and Neurogenic Detrusor overactivity (NDO). Many neurologic pathologies can be responsible of NDO such as Parkinson disease, multiple sclerosis, spinal cord injury, cerebral palsy, brain tumors, cerebrovascular pathologies, and meningomyelocele and the lower urinary tract disfunction depend on the site and the completeness of the associated neurological lesions. Aim of the study is to evaluate preliminary results on symptoms and urodynamic findings of posterior tibial nerve stimulation (PTNS) in patients with NDO.
METHODS: Twelve patients (pts.), 9 men and 3 females, age ranged from 25 and 76 years with clinical and urodynamic diagnosis of NDO were included in the study. Electrical stimulations were applied unilaterally from the medial malleolus and posterior to the edge of the tibia twice a week for a total of 6 weeks. Urodynamic parameters were evaluated before and after treatment. Overactive bladder questionnaire (AOB-q) and voiding diary were performed in all pts. before treatment after 6 elettrical stimulations and at the end of treatment.
RESULTS: PTNS was found to be effective on OAB symtoms in all pts., according to the data of the questionnaires and voiding diary. Mean 1st involuntary detrusor contraction (IDC) and mean maximum cistometric capacity(MCC) were significantly improved on standard cystometry. 8 pts. (66,6%) showed a complete disappearance of IDC and the other 4pts.(33,3%) showed an increased filling volume to IDC. No complications have been reported and pts. compliance to treatment was very high.
CONCLUSIONS: PTNS is an effective and safe option to treat pts. with symptoms from NDO. Further studies are needed to assess the role of this technique, and to establish the length of treatment in order to maintain the results obtained.
Research Center of Pediatric Infectious Diseases, Iran
Title: Dynamic magnetic resonance urography vs DMSA scan, IVP, Ultrasonography in children with urinary tract infection
Time : 14:00-14:30
Samileh Noorbaksh is an Professor Research Center of Pediatric Infectious Diseases, Iran
Background: Urinary tract infection 0 in children causes renal scarring and permanent
damage to the organ. In this study, we compared the diagnostic value of magnetic
resonance urogram for urinary tract anomalies with other conventional imaging
methods in children with UTI.
Methods: In this case-control study, 190 children (mean age 3.23±3.59 yrs) with UTI were recruited from the Pediatric Ward of Rasul-e-Akram Hospital during 2007-2009. The patients were divided into two groups based on the applied imaging technique: MRU (cases) and conventional imaging groups (controls).
Results: Abnormal imaging detection rates for Ultrasonography were 32%, X-ray of kidneys, ureters and bladder (KUB) 9%, Intravenous Pyelogram (IVP) 26%, Voiding Cystoure therogram (VCUG) 54%, Dimercaptosuccinic Acid scan (DMSA) indicating non-obstructive (reflux) uropathy in 76% (mean age 3.5 yrs) and MRU 43% (mean age 1.6 yrs), respectively. A meaningful correlation was observed between MRU and DMSA scan with IVP results (Kappa=0.75). KUB and Ultrasonography had similar results in cases with abnormal MRU and DMSA scan (P=0.121). MRU had strong agreement with VCUG and IVP for the detection of obstructive uropathy and scar due to congenital malformation even during intrauterine life but not with sonography results.
Conclusion: Sonography had poor results for the diagnosis of urinary tract anomalies in comparison with MRU. Use of dynamic MRU for the diagnosis of congenital anomalies (hydronephrosis, obstruction, pyelonephritis, renal scar) in children with UTI seems to be of better help, although higher costs and the need for sedation during MRU still are its disadvantages.